polyclonal rabbit anti-mouse socs3  (Cell Signaling Technology Inc)

Journal: Viruses

Article Title: PCV2 Infection Upregulates SOCS3 Expression to Facilitate Viral Replication in PK-15 Cells

doi: 10.3390/v17081081

Figure Lengend Snippet: PCV2 infection upregulated the mRNA and protein levels of SOCS3 in PK-15 cells. ( a ) PK-15 cells were inoculated with PCV2 at a MOI of 1.0 or mock-infected for 0, 12, 24, and 48 h and analyzed by qRT-PCR measurements of the SOCS3 mRNA. ( b ) Immunoblotting with anti-SOCS3 and anti-β-actin antibodies to measure the SOCS3 protein at 0, 12, 24, and 48 hpi, respectively. Data are presented as mean ± SD ( n = 3). * p < 0.05, *** p < 0.001 vs. control.

Article Snippet: Immunoblotting was performed using a rabbit polyclonal anti-SOCS3 antibody (Proteintech; 1:1000 dilution) incubated overnight at 4 °C, followed by incubation with a HRP-conjugated goat anti-rabbit secondary antibody (Servicebio; 1:3000 dilution) for 1 h at room temperature.

Techniques: Infection, Quantitative RT-PCR, Western Blot, Control

Journal: Viruses

Article Title: PCV2 Infection Upregulates SOCS3 Expression to Facilitate Viral Replication in PK-15 Cells

doi: 10.3390/v17081081

Figure Lengend Snippet: SOCS3 expression facilitates PCV2 replication. ( a ) Fluorescence imaging of PK-15 cells transfected with varying concentrations of Cy3-labeled siRNA. ( b ) SOCS3 mRNA expression levels and ( c ) corresponding knockdown efficiency at different time points post-siRNA transfection. ( d ) Fluorescence visualization of HEK-293T cells at distinct time intervals following lentiviral vector transduction. ( e ) Fluorescence patterns of SOCS3-stably transfected cells and blank controls after 10 consecutive passages. ( f ) Relative SOCS3 mRNA expression levels in stably transfected cells. ( g ) Cell viability analysis of SOCS3-silenced and overexpressing cell lines. ( h ) Protein expression profiles in SOCS3-knockdown and overexpression models. ( i ) Absolute quantitative PCR analysis of PCV2 viral copy numbers in wild-type PK-15, si-NC, and si-SOCS3-2 cells infected with PCV2 (MOI = 1) at indicated time points. ( j ) PCV2 viral load quantification in wild-type PK-15, PK-15-pCDH, and PK-15-SOCS3 cells post-infection. ( k ) Indirect immunofluorescence detection of PCV2 infection status across experimental groups. Data are presented as mean ± SD ( n = 3). *** p < 0.001 vs. control.

Article Snippet: Immunoblotting was performed using a rabbit polyclonal anti-SOCS3 antibody (Proteintech; 1:1000 dilution) incubated overnight at 4 °C, followed by incubation with a HRP-conjugated goat anti-rabbit secondary antibody (Servicebio; 1:3000 dilution) for 1 h at room temperature.

Techniques: Expressing, Fluorescence, Imaging, Transfection, Labeling, Knockdown, Plasmid Preparation, Transduction, Stable Transfection, Over Expression, Real-time Polymerase Chain Reaction, Infection, Immunofluorescence, Control

Journal: Viruses

Article Title: PCV2 Infection Upregulates SOCS3 Expression to Facilitate Viral Replication in PK-15 Cells

doi: 10.3390/v17081081

Figure Lengend Snippet: Effects of SOCS3 on PCV2-induced IFN-β and TNF-α mRNA expressions. ( a ) SOCS3 silencing suppresses IFN-β production in PCV2-infected host cells while ( b ) enhancing TNF-α expression. Relative quantitative PCR analysis of IFN-β and TNF-α mRNA levels in wild-type PK-15, si-NC, and si-SOCS3-2 cells infected with PCV2 (MOI = 1) at indicated time points. ( c ) SOCS3 overexpression promotes IFN-β production in PCV2-infected host cells, whereas ( d ) it inhibits TNF-α expression. Relative quantitative PCR analysis of IFN-β and TNF-α mRNA levels in wild-type PK-15, PK-15-pCDH, and PK-15-SOCS3 cells post-PCV2 infection (MOI = 1). Data are presented as mean ± SD ( n = 3). ** p < 0.01, *** p < 0.001 vs. control.

Article Snippet: Immunoblotting was performed using a rabbit polyclonal anti-SOCS3 antibody (Proteintech; 1:1000 dilution) incubated overnight at 4 °C, followed by incubation with a HRP-conjugated goat anti-rabbit secondary antibody (Servicebio; 1:3000 dilution) for 1 h at room temperature.

Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction, Over Expression, Control

Journal: Neural Regeneration Research

Article Title: Heat shock factor 1 promotes neurite outgrowth and suppresses inflammation in the severed spinal cord of geckos

doi: 10.4103/1673-5374.366495

Figure Lengend Snippet: The primary and secondary antibodies used in western blot assay

Article Snippet: Polyclonal rabbit anti-gecko SOCS3 antibody* , , , , GenScript Biotech Corp , WB.

Techniques: Western Blot

Journal: Immunity

Article Title: Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication

doi: 10.1016/j.immuni.2022.05.013

Figure Lengend Snippet: IL-6 production by cDC1 and cDC2 regulates differential IDO1 expression (A) Gene expression microarray analysis of cDC1 and cDC2 untreated or treated with LPS (250 ng/mL) for 24 h. Bright blue (lowest) to bright red (highest). (B) Heatmap of cytokines in supernatants from cDC1 and cDC2 cultures treated as (A) (n = 2). (C) IL-6-fold induction in LPS (250 ng/mL) stimulated cDC1 and cDC2 over the untreated control (n = 3). (D) IDO1 immunoblot in cDC1 and cDC2 treated as depicted for 48 h (n = 3). (E) SOCS3 immunoblot in cDC1 and cDC2 treated as depicted for 24 h (n = 3). (F) IDO1 immunoblot in cDC1 and cDC2 treated as depicted for 48 h (n = 4). (G) Analysis of skin reactivity is presented as change in footpad weight. n = 6 mice per group (n = 2). (H) EAE score of mixed bone marrow chimeras with bone marrow of indicated genotypes. 6 mice per group, n = 2. Data are shown as means ± SD. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, two-way ANOVA followed by Bonferroni multiple comparison test (C and H) or Tukey’s multiple comparison test (G). β-Actin used as loading control (D–F). Please also see Figure S4 .

Article Snippet: Polyclonal rabbit anti-mouse SOCS3 , Cell Signaling , Cat#: 2923; RRID: AB_2255132.

Techniques: Expressing, Gene Expression, Microarray, Control, Western Blot, Comparison

Journal: Immunity

Article Title: Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication

doi: 10.1016/j.immuni.2022.05.013

Figure Lengend Snippet:

Article Snippet: Polyclonal rabbit anti-mouse SOCS3 , Cell Signaling , Cat#: 2923; RRID: AB_2255132.

Techniques: Produced, Virus, Subcloning, Recombinant, Electron Microscopy, Protease Inhibitor, Purification, Adjuvant, Modification, Selection, Western Blot, In Situ, Enzyme-linked Immunosorbent Assay, Reverse Transcription, SYBR Green Assay, Bradford Protein Assay, Control, Retroviral, Mouse Assay, Sequencing, Real-time Polymerase Chain Reaction, Cloning, Software